Insertion junction: LMJ.RY0402.191007_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.191007
Chromosome:chromosome 3
Location:6126000
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g191800 (1 of 33) PF13855 - Leucine rich repeat (LRR_8) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGTTTACCAGCCATGGGGTTGTACTTTG
Internal bar code:TGGCGTCGGTGGTCGTCAGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:272
LEAP-Seq percent confirming:99.7019
LEAP-Seq n confirming:1338
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGACAATAACGAGGAACG
Suggested primer 2:CTGTGCTCCAACAAGCTCAC