| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191079 |
| Chromosome: | chromosome 10 |
| Location: | 3346728 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443850 | (1 of 1) PTHR11851//PTHR11851:SF136 - METALLOPROTEASE // PROTEIN YHJJ | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCGGGCGCACGTGGGCATGCCCCAGCC |
| Internal bar code: | GGTGGTTTATACAGGCCGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 272 |
| LEAP-Seq percent confirming: | 70.6422 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTTAGGATGCGTGTGTG |
| Suggested primer 2: | GGAGACAGGAGCGAACAGAC |