| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191092 |
| Chromosome: | chromosome 10 |
| Location: | 5226683 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457050 | (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCGGCGCCCCTGAGTTCGGGGCCGTGC |
| Internal bar code: | GGCCACAGAAGGTCAGGGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 99.7579 |
| LEAP-Seq n confirming: | 2060 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCTTCTGTGTTCCTTTGG |
| Suggested primer 2: | AGAGCAGAGCTCTCTGACGG |