| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191095 |
| Chromosome: | chromosome 13 |
| Location: | 1201562 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570000 | HFO2 | Histone H4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGTGGCCGGCAAGCAGGCAATGCCTTCG |
| Internal bar code: | CCCCTAAGGCCATCTCTATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 77.628 |
| LEAP-Seq n confirming: | 1728 |
| LEAP-Seq n nonconfirming: | 498 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGGTTGGTGACATGATTT |
| Suggested primer 2: | AGTTTGGGTTTCGAAGGGTT |