| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191108 |
| Chromosome: | scaffold_19 |
| Location: | 46002 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre19.g750447 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGCAAAGAGTCTGTCATTGAGTCAATGGA |
| Internal bar code: | ATCCTCGTTCCTGGGCGCGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 99.7345 |
| LEAP-Seq n confirming: | 3005 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGACATGGCACATGAAAC |
| Suggested primer 2: | CTCGAGGACTACAACCCAGC |