| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191119 |
| Chromosome: | chromosome_1 |
| Location: | 4391068 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g029750 | FAK2,FAK1,DAAK1 | ODA5-associated flagellar adenylate kinase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGTGTTCCCGAAATACTTCACCCTCAGTC |
| Internal bar code: | GGCCCGGATATAAATGTTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 873 |
| LEAP-Seq percent confirming: | 99.0909 |
| LEAP-Seq n confirming: | 872 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATTTGTGTGCACTGGCAT |
| Suggested primer 2: | AATCCATGCTCCCTTCACAG |