| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.191122 |
| Chromosome: | chromosome 17 |
| Location: | 3042375 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720600 | CAV1 | (1 of 5) PTHR10037//PTHR10037:SF218 - VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM // SUBFAMILY NOT NAMED; Voltage-gated Ca2+ channel, alpha subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATGTTGAGGAGCACGATCATGTGCATT |
| Internal bar code: | TGTCGTAGCTATTATTCCACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 99.609 |
| LEAP-Seq n confirming: | 20125 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACGTCCTGGTACCACTC |
| Suggested primer 2: | CACCAACTGGTTCAGTCCCT |