| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191132 |
| Chromosome: | chromosome 10 |
| Location: | 5607367 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460050 | FLU1,FLU,FLP | (1 of 1) PTHR10098//PTHR10098:SF123 - RAPSYN-RELATED // SUBFAMILY NOT NAMED; FLU protein, chloroplast precursor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGATTTATGCCGGGACACCGGGCATTGT |
| Internal bar code: | TTCCGGGCTGGGATATGGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 693 |
| LEAP-Seq percent confirming: | 99.4913 |
| LEAP-Seq n confirming: | 3716 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGGAGCTGAGCATACA |
| Suggested primer 2: | GTGCAGAGGATGCAGTCTGA |