| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191148 |
| Chromosome: | chromosome 6 |
| Location: | 7429133 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299850 | HLM10 | (1 of 2) K11426 - SET and MYND domain-containing protein (SMYD); Histone-lysine N-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTATGTGCTAGTGAACACTTCGTGTGCG |
| Internal bar code: | GGAAGACGGATGCCCCTGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 73 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGCAAGAAGGGACAAG |
| Suggested primer 2: | ACCTTCATACGCACCCACTC |