| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.191157 |
| Chromosome: | chromosome 16 |
| Location: | 3154453 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665950 | MCM8 | (1 of 1) K10737 - DNA helicase MCM8 [EC:3.6.4.12] (MCM8); Minichromosome maintenance protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCGCACGCCCCCCAGTAGCGCCAGCAC |
| Internal bar code: | TTCGCGAAATCTCCCGATCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 99.8206 |
| LEAP-Seq n confirming: | 1669 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCTACACTCCCGAAAGC |
| Suggested primer 2: | AAGAGTGTGGAGTGTGTGCG |