| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191166 |
| Chromosome: | chromosome 1 |
| Location: | 99318 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000700 | ZFR1 | (1 of 2) PTHR14155//PTHR14155:SF170 - RING FINGER DOMAIN-CONTAINING // SUBFAMILY NOT NAMED; zinc finger protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCCGCTGCCTCTGGCCCCGTTGTCCC |
| Internal bar code: | TTCTGTCAACGGGCGCGGTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 99.5626 |
| LEAP-Seq n confirming: | 2276 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTCTCCACCTCCACTTC |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |