| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.191166 |
| Chromosome: | chromosome 1 |
| Location: | 2863992 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g017250 | FKB99 | Chlorophyceae-specific protein; (1 of 2) K09571 - FK506-binding protein 4/5 (FKBP4_5) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAAATGCAAAATAGCGAAGCGGGAAAT |
| Internal bar code: | CCAGTGGTGCGTAATTTGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 358 |
| LEAP-Seq percent confirming: | 99.5139 |
| LEAP-Seq n confirming: | 4709 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATAGGTGGGGTCCACAAT |
| Suggested primer 2: | GCCTCCTGCTCTTGGTAGTG |