| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.191174 |
| Chromosome: | chromosome 12 |
| Location: | 8701947 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546150 | PETM,PETM1 | Chloroplast cytochrome b6f PetM subunit; (1 of 1) PF08041 - PetM family of cytochrome b6f complex subunit 7 (PetM) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCTATCCTTGCGGGGCGAGGGCCCATC |
| Internal bar code: | ATCTTTGGCGAAGTTAGGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 189 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGGCGCTACAGAAAAGG |
| Suggested primer 2: | GCTGAGCTGGCAAAACTACC |