| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191184 |
| Chromosome: | chromosome 11 |
| Location: | 2824553 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477500 | (1 of 1) PF13664 - Domain of unknown function (DUF4149) (DUF4149) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGAGGCGCGGCCGAGCTCGTTTTGGTTT |
| Internal bar code: | TGGATAGGGAGGGGTGGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 490 |
| LEAP-Seq percent confirming: | 97.828 |
| LEAP-Seq n confirming: | 1126 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCCCTTCCCTCTCATCTC |
| Suggested primer 2: | AGAACTCCACTTCATGCGCT |