| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191208 |
| Chromosome: | chromosome 2 |
| Location: | 599775 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077800 | CSE13,FAP310 | Flagellar Associated Protein 310 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAGTGTCAGAAGTCTTGACTTCCGCCTG |
| Internal bar code: | GTCTGACAGGGTTGACGGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 99.5652 |
| LEAP-Seq n confirming: | 2061 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTTGGGGCGTTTATGTA |
| Suggested primer 2: | AGGTCGGACCCTATGAGCTT |