Insertion junction: LMJ.RY0402.191210_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.191210
Chromosome:chromosome 9
Location:4453048
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g395658 (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCATGCCCATCCTGCCATCCATCCCACCC
Internal bar code:CGCGCGTGCAATTACCCGTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:190
LEAP-Seq percent confirming:98.4439
LEAP-Seq n confirming:1202
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCCGTACAATGTGTCTGTGG
Suggested primer 2:GTGGTCCCATGAAATCTGCT