| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191211 |
| Chromosome: | chromosome_9 |
| Location: | 655138 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g403200 | FAP198 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAACCGAGAGGCCAAGGGCCGCGGGCGCAG |
| Internal bar code: | GGCTGGGAGTTCCGAACCTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 899 |
| LEAP-Seq percent confirming: | 98.9612 |
| LEAP-Seq n confirming: | 3620 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCCACGGACTACTTCAT |
| Suggested primer 2: | CCGTACAAGCCCATCAAAGT |