Insertion junction: LMJ.RY0402.191214_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.191214
Chromosome:chromosome 6
Location:1984700
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g264000 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCTGCGCGCTCCTTCCGCCGTGCCTCA
Internal bar code:GCCCATCGCACGTCCTAACGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:918
LEAP-Seq percent confirming:99.8539
LEAP-Seq n confirming:3418
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTGGTGCCATTTGTGACT
Suggested primer 2:ACTCCAAAGCCACATCCAAC