| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.191230 |
| Chromosome: | chromosome 16 |
| Location: | 4645635 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687500 | ARP2 | Actin-related protein; (1 of 1) K17260 - actin-related protein 2 (ACTR2, ARP2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGAACCATTCCCCCACCACCTTCTTGGC |
| Internal bar code: | AACAGGCCTCTCCGGATCCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 75 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAAGGTCGTCAAGAGCAG |
| Suggested primer 2: | TACGGTATGCTGGGGTCTTC |