Insertion junction: LMJ.RY0402.191263_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.191263
Chromosome:chromosome 17
Location:6008011
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g740700 FAP336 (1 of 7) PF05517 - p25-alpha (p25-alpha); P25 Alpha Domain Containing Flagellar Associated Protein 336 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGGCACACACTCACACCCTGCCACACCC
Internal bar code:TGGCAACTCAGCAGCCGGCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0259
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:3860
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTGTGTGAGTGAGGGG
Suggested primer 2:CTGTGTCCACTGTCCACCAC