| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191265 |
| Chromosome: | chromosome 17 |
| Location: | 6216357 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g742050 | (1 of 18) IPR011051 - RmlC-like cupin domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTAGATTGCGCCGCGACCGTCACGATA |
| Internal bar code: | GGCCGGAAATTGAGAGGCTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 99.8646 |
| LEAP-Seq n confirming: | 1475 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCACACGTACCAGGCTTT |
| Suggested primer 2: | GCTACGGTACGAGCCTTGAG |