Insertion junction: LMJ.RY0402.191288_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.191288
Chromosome:chromosome_6
Location:2107515
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre06.g265200 HFO46,HFO34 Histone H4 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GGCTTCGGCGGCTAAGCGGCTTCTGCGCCG
Internal bar code:TCCGTGCGGGTGTCGGCGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:285
LEAP-Seq percent confirming:99.4798
LEAP-Seq n confirming:2295
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTTGGTGTCCTCGAACAG
Suggested primer 2:GCGACAAATGCAAGTCAGAA