Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.191288 |
Chromosome: | chromosome_6 |
Location: | 2107515 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g265200 | HFO46,HFO34 | Histone H4 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGCTTCGGCGGCTAAGCGGCTTCTGCGCCG |
Internal bar code: | TCCGTGCGGGTGTCGGCGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 285 |
LEAP-Seq percent confirming: | 99.4798 |
LEAP-Seq n confirming: | 2295 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGTTGGTGTCCTCGAACAG |
Suggested primer 2: | GCGACAAATGCAAGTCAGAA |