| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.191305 |
| Chromosome: | chromosome 6 |
| Location: | 5962164 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g288908 | (1 of 30) PTHR24349//PTHR24349:SF79 - SERINE/THREONINE-PROTEIN KINASE // NITROGEN NETWORK KINASE 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACAGCCACAGCCGCGCCAGCAGGCGGCG |
| Internal bar code: | AAATCGCTCGTAAAGGCTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCGAAGTGTCAGTGTCCT |
| Suggested primer 2: | CAACATTGTGTCAGTTCCCG |