| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191308 |
| Chromosome: | chromosome 12 |
| Location: | 2939313 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501703 | (1 of 100) IPR001810 - F-box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGAGGCGATGGGCGAGGTGCCGGAGAG |
| Internal bar code: | GCGCCGAGGAGCTGGGAAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 833 |
| LEAP-Seq percent confirming: | 98.7706 |
| LEAP-Seq n confirming: | 4499 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAAGGGTCCATCAGAAAA |
| Suggested primer 2: | GGTCAGTATAAGGTGCGGGA |