Insertion junction: LMJ.RY0402.191403_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.191403
Chromosome:chromosome 11
Location:465621
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g467592 Probable amino acid/metabolite permease; (1 of 1) PTHR11785//PTHR11785:SF228 - AMINO ACID TRANSPORTER // GABA-SPECIFIC PERMEASE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGTCGGGGTTGCGAGGCAGGTTTCGGGG
Internal bar code:TCGGGACGCGGGATGTCGTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:148
LEAP-Seq percent confirming:99.0944
LEAP-Seq n confirming:2298
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTAGACGGTAGACCAGGG
Suggested primer 2:TGATGATCTGGTTCTGCTGC