| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191416 |
| Chromosome: | chromosome 13 |
| Location: | 5075002 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607150 | CYG57,CYG6,CYG67 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGATGTTTCCTAGCGGATTGCGTGTGGC |
| Internal bar code: | AGCGATGAGAGCGGCTAAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 547 |
| LEAP-Seq percent confirming: | 99.7168 |
| LEAP-Seq n confirming: | 2465 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCTACTTCAAGCGGTTC |
| Suggested primer 2: | AGCACCGCTAGGCTCAAATA |