| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.191422 |
| Chromosome: | chromosome 3 |
| Location: | 9206026 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g213313 | (1 of 2) K00059 - 3-oxoacyl- (fabG) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCATGCCCACGGCAGTCGGCCAATGCC |
| Internal bar code: | TTCATTCGCTCCAAAATCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCGATGTTAGGCAACTG |
| Suggested primer 2: | ACACACACTCCCTGTCCCTC |