| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191449 |
| Chromosome: | chromosome 6 |
| Location: | 4904761 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279250 | PYM1 | (1 of 1) K14294 - partner of Y14 and mago (WIBG, PYM); Partner of Y14/RBM8A-MAGOH | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCAGGACGGCTGCGCCTCCAGACCTCTA |
| Internal bar code: | TAAATCAGCCTGGCGAATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 97.5342 |
| LEAP-Seq n confirming: | 356 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGAGGTGTTGCTTTCAT |
| Suggested primer 2: | AGAACTGGACATGTTTGGGC |