| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191471 |
| Chromosome: | chromosome 3 |
| Location: | 6519598 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g195300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAGCGCTCCACTGATGCTATTGATCGC |
| Internal bar code: | GACGAGCAGCTGCGTTCAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 247 |
| LEAP-Seq percent confirming: | 99.7785 |
| LEAP-Seq n confirming: | 901 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTGCTGTCCAAGAGGTA |
| Suggested primer 2: | TTTGTGCAAGGGTATGGGAT |