Insertion junction: LMJ.RY0402.191474_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.191474
Chromosome:chromosome 16
Location:1359739
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g651900 CRI1,OPR129,OPR126,CRTISO OctotricoPeptide Repeat protein 129 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCCTTCCTCGCGCTGCCGCACGCCTTGC
Internal bar code:GAGTAGCACCCGTGGTGAGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:773
LEAP-Seq percent confirming:99.0205
LEAP-Seq n confirming:2224
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTGTGTGTGTGGTTTCG
Suggested primer 2:CAGTCATTTGCTCGACTCCA