Insertion junction: LMJ.RY0402.191483_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.191483
Chromosome:chromosome 4
Location:1649398
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g213100 CGLD7 conserved, expressed esterase / lipase / thioesterase family protein; (1 of 1) 3.1.1.2 - Arylesterase / Paraoxonase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCAATGCGAGGAAATGTGGGCGGGCGAA
Internal bar code:TCTGAAGACCGCTATAAACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGACCCAGAAAACCAGAA
Suggested primer 2:TTCAGCCTTATACCCCAACG