Insertion junction: LMJ.RY0402.191505_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.191505
Chromosome:chromosome 17
Location:1906048
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g710300 PHC31 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 31 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGGACACGGTGTAGAGCGGGTCCAAACG
Internal bar code:CGGGGGAGTGATGCCGACGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1004
LEAP-Seq percent confirming:98.4995
LEAP-Seq n confirming:3151
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAGGGCGTCAACAATGTA
Suggested primer 2:GCCTTGAAAGAAGTTGCCAG