| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.191533 |
| Chromosome: | chromosome 7 |
| Location: | 6354732 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGTGCCCCACCTGCGCCGTTCACTCCG |
| Internal bar code: | AGAAAGGTAGTTTGGGCCAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 99.6783 |
| LEAP-Seq n confirming: | 2169 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTCCAACGTTTGTTACGA |
| Suggested primer 2: | GTAGGGAGAAGGATGGGGAG |