| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191549 |
| Chromosome: | chromosome 1 |
| Location: | 600247 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003475 | (1 of 2) 3.4.24.35 - Gelatinase B / Type V collagenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGCATCCTTACCACCCATCCTGCCTGCC |
| Internal bar code: | CTCTCCCGAACCGTGTGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 158 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 108 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTACGTCCTGCCCTCAT |
| Suggested primer 2: | GGTGGTTCAGCCTTCATCAT |