| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.191554 |
| Chromosome: | chromosome 10 |
| Location: | 5856449 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461750 | DMC5 | Putative DNA methylase; (1 of 1) PF00145//PF12047 - C-5 cytosine-specific DNA methylase (DNA_methylase) // Cytosine specific DNA methyltransferase replication foci domain (DNMT1-RFD) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCCGCAGCGGCTGCCATCAAGGACAAGA |
| Internal bar code: | AGTTTCTCCTCGGGTAGCAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 285 |
| LEAP-Seq percent confirming: | 98.0303 |
| LEAP-Seq n confirming: | 647 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTACGCACCTTGACAGTG |
| Suggested primer 2: | ACCAAACCAAGCCAAACAAG |