| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191565 |
| Chromosome: | chromosome 12 |
| Location: | 3919423 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516100 | (1 of 1) PF04061 - ORMDL family (ORMDL) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAACCGTCTCCAACATAGAAAGCCCACG |
| Internal bar code: | GGGCGGTACCCGCCTGATTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1004 |
| LEAP-Seq percent confirming: | 99.9052 |
| LEAP-Seq n confirming: | 3163 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGCAGGCTGTGAACGTA |
| Suggested primer 2: | CTTCTGGGAGCAGGTAGACG |