| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.191572 |
| Chromosome: | chromosome 11 |
| Location: | 1490505 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467742 | (1 of 3) PF04434 - SWIM zinc finger (SWIM) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACACGAGGTGAGCTCCCCACACACACAC |
| Internal bar code: | GGGGCCGAACGCCAGTGCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 99.5868 |
| LEAP-Seq n confirming: | 6267 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACTGCAAGCACATCAAG |
| Suggested primer 2: | CGACCGGACTCTCCTTGTAA |