| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191584 |
| Chromosome: | chromosome 8 |
| Location: | 4209965 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380400 | MAD2 | (1 of 1) K02537 - mitotic spindle assembly checkpoint protein MAD2 (MAD2); Mitotic spindle assembly checkpoint protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCTGGCATTCTGCGCCAGGTGCGGAGG |
| Internal bar code: | TAGGGAGCTTTAAAAGGTATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 743 |
| LEAP-Seq percent confirming: | 99.7458 |
| LEAP-Seq n confirming: | 2747 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCAAAGGTGAGTCCACG |
| Suggested primer 2: | CACACCAGCATAACCACCAG |