| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.191587 |
| Chromosome: | chromosome 14 |
| Location: | 1403614 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617550 | ODA-DC3,ODA14,DC3,DLE3 | (1 of 1) PTHR10891//PTHR10891:SF600 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED; Outer Arm Dynein Docking Complex 3 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCGCACGTTTTGTAATGTCAGCAGTCCA |
| Internal bar code: | TAACCCACGATTTTCTCTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1046 |
| LEAP-Seq percent confirming: | 99.3586 |
| LEAP-Seq n confirming: | 1549 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGGTTGACTGGTGATGA |
| Suggested primer 2: | CCCTGGTTGTTCTTGTCGTT |