| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.191596 |
| Chromosome: | chromosome 1 |
| Location: | 371563 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002250 | NAT4 | (1 of 1) PTHR23091//PTHR23091:SF206 - N-TERMINAL ACETYLTRANSFERASE // ACYL-COA N-ACYLTRANSFERASE DOMAIN-CONTAINING PROTEIN; Histone acetyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAGGCATCGGAGGCGCACTGCGCGCACA |
| Internal bar code: | GTCATAGTCCTGGGACTTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 77.3298 |
| LEAP-Seq n confirming: | 1170 |
| LEAP-Seq n nonconfirming: | 343 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGTTTGGTTTACAGCG |
| Suggested primer 2: | CCTCACCATCAGTTTCCGTT |