| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.191603 |
| Chromosome: | chromosome 12 |
| Location: | 8226539 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550468 | intron | ||
| Cre12.g550501 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTAAAACCTGGAGCTCAGGACCTGTGCC |
| Internal bar code: | CATTCGCGGGCGCCTCAAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 98.6 |
| LEAP-Seq n confirming: | 986 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACTACGTGGCACGCTTAC |
| Suggested primer 2: | GGGAATTGCCTGGTCTTGTA |