| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.191612 |
| Chromosome: | chromosome 9 |
| Location: | 3211460 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387541 | (1 of 33) IPR015797 - NUDIX hydrolase domain-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAACACTCGTCAACCAGCTTCCCATCAC |
| Internal bar code: | ACCTCCCATCGAGGCTGCGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 257 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 177 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCCTTTAATCACCGTGT |
| Suggested primer 2: | CGCATTTTGGTTTCTTGGAT |