| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191642 |
| Chromosome: | chromosome_14 |
| Location: | 355275 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g610200 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGCTGGCGCCATCGGCGCTGCGGTCGCGC |
| Internal bar code: | CCACCCGGCCCGTCGGTATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 204 |
| LEAP-Seq percent confirming: | 55.9372 |
| LEAP-Seq n confirming: | 1140 |
| LEAP-Seq n nonconfirming: | 898 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAACAACATCAGCAGGA |
| Suggested primer 2: | AGGCCTAGGAGTTGGATCGT |