| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191644 |
| Chromosome: | chromosome 2 |
| Location: | 5421450 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107450 | ATS2 | ATP-sulfurylase; (1 of 2) 2.7.7.4 - Sulfate adenylyltransferase / Sulfurylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAAGCATCCTGTTGCTGCTTAAGGGTCT |
| Internal bar code: | TGCTGGCAGCCCGGCTAGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 99.1169 |
| LEAP-Seq n confirming: | 3367 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGTACAGCGGTAGATCG |
| Suggested primer 2: | TTTCCCCTCACGCTCTTCTA |