| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191666 |
| Chromosome: | chromosome 2 |
| Location: | 509843 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g076987 | (1 of 10) PTHR31867//PTHR31867:SF1 - FAMILY NOT NAMED // EXPANSIN-A1-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTATCGAGCTACTTTATGCCACACGCTT |
| Internal bar code: | CGGGTGAGCATGTTGTGGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 99.8434 |
| LEAP-Seq n confirming: | 2550 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTTCTTTCAAGCACATC |
| Suggested primer 2: | CATAAACACGGTGCAGGTTG |