| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191671 |
| Chromosome: | chromosome 12 |
| Location: | 5231788 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528200 | (1 of 1) K10742 - DNA replication ATP-dependent helicase Dna2 (DNA2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGACAAGGCGCTAGCGGCGCAGGTGTCT |
| Internal bar code: | CACTGGTACCTCGGCCGTAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 99.8369 |
| LEAP-Seq n confirming: | 2449 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCATAGCGAGCGAGGTC |
| Suggested primer 2: | CTGTTGACTAGCGCATTGGA |