| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.191679 |
| Chromosome: | chromosome 10 |
| Location: | 2125448 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433450 | (1 of 1) PTHR22844//PTHR22844:SF125 - F-BOX AND WD40 DOMAIN PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGCGCACAGGCCGTATACACATGCACA |
| Internal bar code: | CAGTCCGGCGCTCGCCCTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 381 |
| LEAP-Seq percent confirming: | 99.8268 |
| LEAP-Seq n confirming: | 1153 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGTGTGTCTATGTGCGT |
| Suggested primer 2: | AGGTGGAGTGACTCGCTGTT |