Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.191727 |
Chromosome: | chromosome_13 |
Location: | 3743268 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g589450 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCGCACCCACGTCCTGAGCCTGGGCATCGG |
Internal bar code: | CCAAAGTTGGCCATGGGCTTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 951 |
LEAP-Seq percent confirming: | 95.4545 |
LEAP-Seq n confirming: | 21 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGGCTCAGAGCTAACCACC |
Suggested primer 2: | TTGTCACTGTAGCGCCACTC |