| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191764 |
| Chromosome: | chromosome 12 |
| Location: | 293070 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485600 | SNRK2M | (1 of 4) PTHR24343//PTHR24343:SF167 - SERINE/THREONINE KINASE // SUBFAMILY NOT NAMED; snRK1 family in Chlamydomonas, subgroup 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCAATGGAACAGTTGAAGTCTGCGCCG |
| Internal bar code: | ATGCCGCAGGCTTAGATCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 75.2898 |
| LEAP-Seq n confirming: | 1624 |
| LEAP-Seq n nonconfirming: | 533 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTCGGTTCATTTCCTCC |
| Suggested primer 2: | TTGGGATGGAGAGCCACTAC |