Insertion junction: LMJ.RY0402.191797_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.191797
Chromosome:chromosome 6
Location:1608625
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g260700 XUV1,UAPA6 (1 of 2) K06901 - putative MFS transporter, AGZA family, xanthine/uracil permease (pbuG); Xanthine/uracil/vitamin C permease-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGTACACACACGGCAGCAGCTTGAAAGA
Internal bar code:TGTTCTCCAGGTACCCTAGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:185
LEAP-Seq percent confirming:95.4023
LEAP-Seq n confirming:166
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAGCTTGAAGTGACGAAG
Suggested primer 2:CTTGTGCGCTATGAGGTCAA